PFI-3 induces vasorelaxation with potency to reduce extracellular calcium influx in rat mesenteric artery

ABSTRACT:

Background

PFI-3 is a small-molecule inhibitor that targets the bromodomains (BRDs) of Brahma-related gene 1 (BRG1). This monomeric compound, which has high selectivity and potent cellular effects, has recently been developed. Although PFI-3 has been reported as a potential therapeutic agent targeting thrombomodulin, its role in the regulation of vascular function remains unknown. Therefore, we aimed to investigate the impact of PFI-3 on arterial vessel tone.

Methods

A microvascular tension measurement device (DMT) was utilized to identify alterations in vascular tension within the mesenteric artery. To detect variations in cytosolic [Ca2+]i, a Fluo-3/AM fluorescent probe and fluorescence microscope were employed. Additionally, whole-cell patch clamp techniques were utili

SUBMITTER: Li J 

PROVIDER: S-EPMC10225122 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature