Fibronectin extra domain a limits liver dysfunction and protects mice during acute inflammation.

ABSTRACT:

Background and aim

The primary transcript of fibronectin (FN) undergoes alternative splicing to generate different isoforms, including FN containing the Extra Domain A (FN_EDA+), whose expression is regulated spatially and temporarily during developmental and disease conditions including acute inflammation. The role of FN_EDA+ during sepsis, however, remains elusive.

Methods

Mice constitutively express the EDA domain of fibronectin (EDA^+/+); lacking the FN EDA domain (EDA^-/-) or with a conditional ablation of EDA + inclusion only in liver produced FN (alb-CRE⁺EDA floxed mice) thus expressing normal plasma FN were used. Systemic inflammation and sepsis were induced by either LPS injection (70 mg/kg) or by cecal ligation and puncture (CLP) Neut

SUBMITTER: Venu VKP 

PROVIDER: S-EPMC10242638 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature