Abnormally elevated expression of ACTA2 of circular smooth muscle leads to hyperactive contraction in aganglionic segments of HSCR.

ABSTRACT:

Background

Actin Alpha 2 (ACTA2) is expressed in intestinal smooth muscle cells (iSMCs) and is associated with contractility. Hirschsprung disease (HSCR), one of the most common digested tract malformations, shows peristaltic dysfunction and spasm smooth muscles. The arrangement of the circular and longitudinal smooth muscle (SM) of the aganglionic segments is disorganized. Does ACTA2, as a marker of iSMCs, exhibit abnormal expression in aganglionic segments? Does the ACTA2 expression level affect the contraction function of iSMCs? What are the spatiotemporal expression trends of ACTA2 during different developmental stages of the colon?

Methods

Immunohistochemical staining was used to detect the expression of ACTA2 in iSMCs of children with HSCR and Ednrb^-/- mice,

SUBMITTER: Chen K 

PROVIDER: S-EPMC10244273 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature