B cell profiles, antibody repertoire and reactivity reveal dysregulated responses with autoimmune features in melanoma.

ABSTRACT: B cells are known to contribute to the anti-tumor immune response, especially in immunogenic tumors such as melanoma, yet humoral immunity has not been characterized in these cancers to detail. Here we show comprehensive phenotyping in samples of circulating and tumor-resident B cells as well as serum antibodies in melanoma patients. Memory B cells are enriched in tumors compared to blood in paired samples and feature distinct antibody repertoires, linked to specific isotypes. Tumor-associated B cells undergo clonal expansion, class switch recombination, somatic hypermutation and receptor revision. Compared with blood, tumor-associated B cells produce antibodies with proportionally higher levels of unproductive sequences and distinct complementarity determining region 3 properties. The obs

SUBMITTER: Crescioli S 

PROVIDER: S-EPMC10249578 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature