Generation of Synthetic Acinetobacter baumannii-Specific Nanobodies.

ABSTRACT: The bacterial pathogen Acinetobacter baumannii is a leading cause of drug-resistant infections. Here, we investigated the potential of developing nanobodies that can recognize A. baumannii over other Gram-negative bacteria. Through generation and panning of a synthetic nanobody library, we identified several potential lead candidates. We demonstrate how incorporation of next-generation sequencing analysis can aid in the selection of lead candidate nanobodies. Using monoclonal phage display, we validated the binding of lead nanobodies to A. baumannii. Subsequent purification and biochemical characterization revealed one particularly robust nanobody that specifically bound select A. baumannii strains compared to other common drug-resistant pathogens. These finding

SUBMITTER: Knauf GA 

PROVIDER: S-EPMC10262196 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature