Single-cell and spatial transcriptome analysis reveals the cellular heterogeneity of liver metastatic colorectal cancer.

ABSTRACT: In this study, we comprehensively charted the cellular landscape of colorectal cancer (CRC) and well-matched liver metastatic CRC using single-cell and spatial transcriptome RNA sequencing. We generated 41,892 CD45^- nonimmune cells and 196,473 CD45⁺ immune cells from 27 samples of six CRC patients, and found that CD8_CXCL13 and CD4_CXCL13 subsets increased significantly in liver metastatic samples that exhibited high proliferation ability and tumor-activating characterization, contributing to better prognosis of patients. Distinct fibroblast profiles were observed in primary and liver metastatic tumors. F3⁺ fibroblasts enriched in primary tumors contributed to worse overall survival by expressing protumor factors. However, MCAM⁺ fib

SUBMITTER: Wang F 

PROVIDER: S-EPMC10275599 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature