Unbiased Virus Detection in a Danish Zoo Using a Portable Metagenomic Sequencing System.

ABSTRACT: Metagenomic next-generation sequencing (mNGS) is receiving increased attention for the detection of new viruses and infections occurring at the human-animal interface. The ability to actively transport and relocate this technology enables in situ virus identification, which could reduce response time and enhance disease management. In a previous study, we developed a straightforward mNGS procedure that greatly enhances the detection of RNA and DNA viruses in human clinical samples. In this study, we improved the mNGS protocol with transportable battery-driven equipment for the portable, non-targeted detection of RNA and DNA viruses in animals from a large zoological facility, to simulate a field setting for point-of-incidence virus detection. From the resulting metagenomic data, we detecte

SUBMITTER: Fomsgaard AS 

PROVIDER: S-EPMC10303153 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature