Dataset Information

Human ovarian gross morphology and subanatomy across puberty: insights from tissue donated during fertility preservation.

ABSTRACT:

Objective

To study ovarian gross morphologic and subanatomic features across pubertal development.

Design

Prospective cohort study.

Setting

An academic medical center with specimens collected from 2018-2022.

Patients

Tissue was obtained from prepubertal and postpubertal participants (0.19-22.96 years) undergoing ovarian tissue cryopreservation before treatment that put them at a significantly or high increased risk of developing premature ovarian insufficiency. Most participants (64%) had not received chemotherapy at tissue collection.

Interventions

None.

Main outcome measures

Ovaries procured for fertility preservation were weighed and measured. Ovarian tissue fragments released during processing, biopsies used for pathology, and hormone panels were analyzed for gross morphology, subanatomic features, and reproductive hormones. Graphical analysis of best-fit lines determined age at maximum growth velocity.

Results

Prepubertal ovaries were significantly (1.4-fold and 2.4-fold) smaller than postpubertal ovaries by length and width and 5.7-fold lighter on average. Length, width, and weight grew in a sigmoidal pattern with age. Prepubertal ovaries were less likely to display a defined corticomedullary junction (53% vs. 77% in postpubertal specimens), less likely to have a tunica albuginea (22% vs. 93% in postpubertal specimens), contained significantly more (9.8-fold) primordial follicles, and contained primordial follicles at significantly deeper depths (2.9-fold) when compared with postpubertal ovaries.

Conclusions

Ovarian tissue cryopreservation is a resource to study human ovarian biology and pubertal development. Maximum growth velocity occurs late within the pubertal transition (Tanner 3+) after changes in subanatomic features. This ovarian morphology model adds to foundational knowledge of human ovarian development and supports ongoing transcriptomics research.

SUBMITTER: Tsui EL

PROVIDER: S-EPMC10310944 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Human ovarian gross morphology and subanatomy across puberty: insights from tissue donated during fertility preservation.

Tsui Elizabeth L EL Harris Courtney J CJ Rowell Erin E EE Laronda Monica M MM

F&S reports 20230223 2

<h4>Objective</h4>To study ovarian gross morphologic and subanatomic features across pubertal development.<h4>Design</h4>Prospective cohort study.<h4>Setting</h4>An academic medical center with specimens collected from 2018-2022.<h4>Patients</h4>Tissue was obtained from prepubertal and postpubertal participants (0.19-22.96 years) undergoing ovarian tissue cryopreservation before treatment that put them at a significantly or high increased risk of developing premature ovarian insufficiency. Most ...[more]

PMID: 37398615

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Dataset Information

Human ovarian gross morphology and subanatomy across puberty: insights from tissue donated during fertility preservation.

Objective

Design

Setting

Patients

Interventions

Main outcome measures

Results

Conclusions

Publications

Human ovarian gross morphology and subanatomy across puberty: insights from tissue donated during fertility preservation.

Similar Datasets

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