Mapping alternative polyadenylation in human cells using direct RNA sequencing technology.
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ABSTRACT:
SUBMITTER: Polenkowski M
PROVIDER: S-EPMC10362186 | biostudies-literature | 2023 Jul
REPOSITORIES: biostudies-literature
Publications
Mapping alternative polyadenylation in human cells using direct RNA sequencing technology.
Polenkowski Mareike M Allister Aldrige Bernardus AB Burbano de Lara Sebastian S Soltau Madleen M Kendre Gajanan G Tran Doan Duy Hai DDH
STAR protocols 20230710 3
Alternative cleavage and polyadenylation (APA) is a widespread mechanism to generate mRNA isoforms with alternative 3' untranslated regions. Here, we detail a protocol for detecting APA genome wide using direct RNA sequencing technology including computational analysis. We describe steps for RNA sample and library preparation, nanopore sequencing, and data analysis. Experiments and data analysis can be performed over a period of 6-8 days and require molecular biology and bioinformatics skills. F ...[more]
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