A modular chemigenetic calcium indicator enables in vivo functional imaging with near-infrared light.

ABSTRACT: Genetically encoded fluorescent calcium indicators have revolutionized neuroscience and other biological fields by allowing cellular-resolution recording of physiology during behavior. However, we currently lack bright, genetically targetable indicators in the near infrared that can be used in animals. Here, we describe WHaloCaMP, a modular chemigenetic calcium indicator built from bright dye-ligands and protein sensor domains that can be genetically targeted to specific cell populations. Fluorescence change in WHaloCaMP results from reversible quenching of the bound dye via a strategically placed tryptophan. WHaloCaMP is compatible with rhodamine dye-ligands that fluoresce from green to near-infrared, including several dye-ligands that efficiently label the central nervous system in anima

SUBMITTER: Farrants H 

PROVIDER: S-EPMC10370049 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature