Full thickness 3D in vitro conjunctiva model enables goblet cell differentiation.

ABSTRACT: In vitro culture and generation of highly specialized goblet cells is still a major challenge in conjunctival 3D in vitro equivalents. A model comprising all physiological factors, including mucus-secreting goblet cells has the potential to act as a new platform for studies on conjunctival diseases. We isolated primary conjunctival epithelial cells and fibroblasts from human biopsies. 3D models were generated from either epithelial layers or a combination of those with a connective tissue equivalent. Epithelial models were investigated for marker expression and barrier function. Full-thickness models were analyzed for goblet cell morphology and marker expression via immunofluorescence and quantitative real-time PCR. Simple epithelial models cultured at the air-liquid interface showed strat

SUBMITTER: Schwebler J 

PROVIDER: S-EPMC10382544 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature