Characterization of cell-fate decision landscapes by estimating transcription factor dynamics.

ABSTRACT: Time-specific modulation of gene expression during differentiation by transcription factors promotes cell diversity. However, estimating their dynamic regulatory activity at the single-cell level and in a high-throughput manner remains challenging. We present FateCompass, an integrative approach that utilizes single-cell transcriptomics data to identify lineage-specific transcription factors throughout differentiation. By combining a probabilistic framework with RNA velocities or differentiation potential, we estimate transition probabilities, while a linear model of gene regulation is employed to compute transcription factor activities. Considering dynamic changes and correlations of expression and activities, FateCompass identifies lineage-specific regulators. Our validation using in

SUBMITTER: Jimenez S 

PROVIDER: S-EPMC10391345 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature