Novel positron emission tomography imaging targeting cell surface glycans for pancreatic cancer: ¹⁸ F-labeled rBC2LCN lectin.

ABSTRACT: Advancement in early detection modalities will greatly improve the overall prognoses of pancreatic ductal adenocarcinoma (PDAC). For this purpose, we report a novel class of tumor-specific probes for positron emission tomography (PET) based on targeting cell surface glycans. The PDAC-targeting ability of rBC2LCN lectin, combined with fluorine-18 (¹⁸ F) ([¹⁸ F]FB-rBC2LCN), resulted in reproducible, high-contrast PET imaging of tumors in a PDAC xenograft mouse model. [¹⁸ F]N-succinimidyl-4-fluorobenzoate ([¹⁸ F]SFB) was conjugated to rBC2LCN, and [¹⁸ F]FB-rBC2LCN was successfully prepared with a radiochemical purity >95%. Cell binding and uptake revealed that [¹⁸ F]FB-rBC2LCN binds to H-type-3-positive Capan-1 pancreatic cance

SUBMITTER: Kuroda Y 

PROVIDER: S-EPMC10394132 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature