Enhanced differentiation of the mouse oli-neu oligodendroglial cell line using optimized culture conditions.

ABSTRACT:

Objective

Oligodendrocytes (OL) are the glial cell type in the CNS that are responsible for myelin formation. The ability to culture OLs in vitro has provided critical insights into the mechanisms underlying their function. However, primary OL cultures are tedious to obtain, difficult to propagate and are not easily conducive to genetic manipulation. To overcome these obstacles, researchers have generated immortalized OL like cell lines derived from various species. One such cell line is the mouse Oli-neu line which is thought to recapitulate characteristics of OLs in early stages of maturity. They have been extensively utilized in multiple studies as surrogates for OLs, especially in analyzing epigenetic modifications and regulatory pathways in the OL lineage.

Results

In th

SUBMITTER: Bey GR 

PROVIDER: S-EPMC10401818 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature