Screening bisphenols in complex samples via a planar Arxula adeninivorans bioluminescence bioassay.

ABSTRACT: The Arxula yeast bisphenol screen (A-YBS) utilizes the bioluminescent Arxula adeninivorans yeast-based reporter cells for tailored analysis of bisphenols, one of the major endocrine-disrupting compound groups. For the first time, this bioreporter has been applied on the high-performance thin-layer chromatography (HPTLC) adsorbent surface to develop a respective planar bioluminescence bioassay (pA-YBS). The goal was to combine the advantages of HPTLC with a more selective bioassay detection for bisphenols. The performance of this pA-YBS bioluminescence bioassay was demonstrated by calculating the half-maximal effective concentration (EC₅₀) of bisphenols compared to references. The EC₅₀ ranged from 267 pg/band for bisphenol Z and 322 pg/band for bisphenol A (BPA) to > 1

SUBMITTER: Jaber M 

PROVIDER: S-EPMC10404207 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature