Utility of Protein Microarrays for Detection of Classified and Novel Antibodies in Autoimmune Neurologic Disease.

ABSTRACT:

Background and objectives

Neural antibodies are detected by tissue-based indirect immunofluorescence assay (IFA) in Mayo Clinic's Neuroimmunology Laboratory practice, but the process of characterizing and validating novel antibodies is lengthy. We report our assessment of human protein arrays.

Methods

Assessment of arrays (81% human proteome coverage) was undertaken using diverse known positive samples (17 serum and 14 CSF). Samples from patients with novel neural antibodies were reflexed from IFA to arrays. Confirmatory assays were cell-based (CBA) or line blot. Epitope mapping was undertaken using phage display immunoprecipitation sequencing (PhiPSeq).

Results

Control positive samples known to be reactive with linear epitopes of intracellular antigens (e.g., ANNA-1

SUBMITTER: McKeon A 

PROVIDER: S-EPMC10406426 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature