Specific detection of tau seeding activity in Alzheimer's disease using rationally designed biosensor cells.

ABSTRACT:

Background

The prion-like propagation of tau in neurodegenerative disorders implies that misfolded pathological tau can recruit the normal protein and template its aggregation. Here, we report the methods for the development of sensitive biosensor cell lines for the detection of tau seeding activity.

Results

We performed the rational design of novel tau probes based on the current structural knowledge of pathological tau aggregates in Alzheimer's disease. We generated Förster resonance energy transfer (FRET)-based biosensor stable cell lines and characterized their sensitivity, specificity, and overall ability to detect bioactive tau in human samples. As compared to the reference biosensor line, the optimized probe design resulted in an increased efficiency in the detection

SUBMITTER: Lathuiliere A 

PROVIDER: S-EPMC10408046 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature