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Efficient Metabolic Fingerprinting of Follicular Fluid Encodes Ovarian Reserve and Fertility.


ABSTRACT: Ovarian reserve (OR) and fertility are critical in women's healthcare. Clinical methods for encoding OR and fertility rely on the combination of tests, which cannot serve as a multi-functional platform with limited information from specific biofluids. Herein, metabolic fingerprinting of follicular fluid (MFFF) from follicles is performed, using particle-assisted laser desorption/ionization mass spectrometry (PALDI-MS) to encode OR and fertility. PALDI-MS allows efficient MFFF, showing fast speed (≈30 s), high sensitivity (≈60 fmol), and desirable reproducibility (coefficients of variation <15%). Further, machine learning of MFFF is applied to diagnose diminished OR (area under the curve of 0.929) and identify high-quality oocytes/embryos (p < 0.05) by a single PALDI-MS test. Meanwhile, metabolic biomarkers from MFFF are identified, which also determine oocyte/embryo quality (p < 0.05) from the sampling follicles toward fertility prediction in clinics. This approach offers a powerful platform in women's healthcare, not limited to OR and fertility.

SUBMITTER: Wu J 

PROVIDER: S-EPMC10427401 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature

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Efficient Metabolic Fingerprinting of Follicular Fluid Encodes Ovarian Reserve and Fertility.

Wu Jiao J   Liang Chunmei C   Wang Xin X   Huang Yida Y   Liu Wanshan W   Wang Ruimin R   Cao Jing J   Su Xun X   Yin Tao T   Wang Xiaolei X   Zhang Zhikang Z   Shen Lingchao L   Li Danyang D   Zou Weiwei W   Wu Ji J   Qiu Lihua L   Di Wen W   Cao Yunxia Y   Ji Dongmei D   Qian Kun K  

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 20230613 23


Ovarian reserve (OR) and fertility are critical in women's healthcare. Clinical methods for encoding OR and fertility rely on the combination of tests, which cannot serve as a multi-functional platform with limited information from specific biofluids. Herein, metabolic fingerprinting of follicular fluid (MFFF) from follicles is performed, using particle-assisted laser desorption/ionization mass spectrometry (PALDI-MS) to encode OR and fertility. PALDI-MS allows efficient MFFF, showing fast speed  ...[more]

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