Estimation of skeletal muscle mass in 4-year-old children using the D₃-creatine dilution method.

ABSTRACT:

Background

Given limited experience in applying the creatine-(methyl-D₃) (D₃Cr) dilution method to measure skeletal muscle mass (SMM) in young children, the feasibility of deployment in a fielding setting and performance of the method was assessed in a cohort of 4-year-old children in Dhaka, Bangladesh.

Methods

Following D₃Cr oral dose (10 mg) administration, single fasting urine samples were collected at 2-4 days (n = 100). Twenty-four-hour post-dose collections and serial spot urine samples on days 2, 3 and 4 were obtained in a subset of participants (n = 10). Urinary creatine, creatinine, D₃Cr and D₃-creatinine enrichment were analyzed by liquid chromatography-tandem mass spectrometry. Appendicular lean mass (ALM) was

SUBMITTER: Sidiqi A 

PROVIDER: S-EPMC10444613 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature