Dataset Information

Targeted regulation and optimization of multifunctional phase transition materials by novel void occupancy engineering† † Electronic supplementary information (ESI) available: Experimental section, Fig. S1–S14, and Tables S1–S11. CCDC 2265083–2265089. For ESI and crystallographic data in CIF or other electronic format see DOI: https://doi.org/10.1039/d3sc02652c

ABSTRACT: As an innovative form of stimulus-response materials, organic–inorganic hybrid phase transition materials have become a wonderful contender in the field of functional electronic equipment due to their versatile structure, intensive functions and straightforward preparation. However, the targeted regulation and optimization of the electrical/optical response, along with the establishment of regular structure–performance relationships, pose significant challenges in meeting the diverse demands of practical applications over an extended period. Herein, we conducted a systematic investigation into the role of lattice void occupancy in regulating phase transition temperature (Tp) and related optical/electrical bistability. By taking hybrid material [TMEA][Cd(SCN)3] featuring a flexible ammonium cation [TMEA]+ (TMEA = ethyltrimethylammonium) as the prototype, we successfully synthesized three phase transition materials, namely [DEDMA][Cd(SCN)3], [TEMA][Cd(SCN)3] and [TEA][Cd(SCN)3] (DEDMA = diethyldimethylammonium, TEMA = triethylmethylammonium, and TEA = tetraethylammonium), and the excellent regulation of the physical properties of these compounds was achieved through subtle engineering of void occupancy. More strikingly, [TEA][Cd(SCN)3] exhibits remarkable bistable properties in terms of dielectric and nonlinear optical responses (with second-harmonic generation intensity reaching 2.5 times that of KDP). This work provides a feasible avenue to reasonably customise organic–inorganic hybrid phase transition materials and finely adjust their intriguing functionalities. Similar to a tossed stone raising a thousand ripples, it was found that a small structural mechanism associated with void occupancy has subtle influence on phase transition material physical properties in this work.

SUBMITTER: Wang Z

PROVIDER: S-EPMC10466303 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature

ACCESS DATA

Similar Datasets

Project description:BackgroundIn the enzymatic conversion of biomass, it becomes an important issue to efficiently and cost-effectively degrade cellulose into fermentable glucose. β-Glucosidase (Bgluc), an essential member of cellulases, plays a critical role in cellulosic biomass degradation. The difficulty in improving the stability of Bgluc has been a bottleneck in the enzyme-dependent cellulose degradation. The traditional method of protein purification, however, leads to higher production cost and a decrease in activity. To simplify and efficiently purify Bgluc with modified special properties, Bgluc-tagged ELP and His with defined phase transitions was designed to facilitate the process.ResultsHere, a novel binary ELP and His tag was fused with Bgluc from termite Coptotermes formosanus to construct a Bgluc-linker-ELP-His recombinant fusion protein (BglucLEH). The recombinant plasmid Bgluc expressing a His tag (BglucH) was also constructed. The BglucLEH and BglucH were expressed in E. coli BL21 and purified using inverse transition cycling (ITC) or Ni-NTA resin. The optimum salt concentration for the ITC purification of BglucLEH was 0.5 M (NH4)2SO4 and the specific activity of BglucLEH purified by ITC was 75.5 U/mg for substrate p-NPG, which was slightly higher than that of BglucLEH purified by Ni-NTA (68.2 U/mg). The recovery rate and purification fold of BglucLEH purified by ITC and Ni-NTA were 77.8%, 79.1% and 12.60, 11.60, respectively. The results indicated that purification with ITC was superior to the traditional Ni-NTA. The K m of BglucLEH and BglucH for p-NPG was 5.27 and 5.73 mM, respectively. The K ca t/K m (14.79 S-1 mM-1) of BglucLEH was higher than that of BglucH (12.10 S-1 mM-1). The effects of ELP tag on the enzyme activity, secondary structure and protein stability were also studied. The results showed that ELP tag did not affect the secondary structure or enzyme activity of Bgluc. More importantly, ELP improved the protein stability in harsh conditions such as heating and exposure to denaturant.ConclusionThe Bgluc-linker-ELP-His system shows wide application prospect in maintaining the activity, efficient purification and improving the stability of Bgluc. These properties of BglucLEH make it an interesting tool to reduce cost, to improve the efficiency of biocatalyst and potentially to enhance the degradation of lignocellulosic biomass.

Dataset Information

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets