Mitochondrial sodium/calcium exchanger NCLX regulates glycolysis in astrocytes, impacting on cognitive performance.

ABSTRACT: Intracellular Ca²⁺ concentrations are strictly controlled by plasma membrane transporters, the endoplasmic reticulum, and mitochondria, in which Ca²⁺ uptake is mediated by the mitochondrial calcium uniporter complex (MCUc), while efflux occurs mainly through the mitochondrial Na⁺ /Ca²⁺ exchanger (NCLX). RNAseq database repository searches led us to identify the Nclx transcript as highly enriched in astrocytes when compared with neurons. To assess the role of NCLX in mouse primary culture astrocytes, we inhibited its function both pharmacologically or genetically. This resulted in re-shaping of cytosolic Ca²⁺ signaling and a metabolic shift that increased glycolytic flux and lactate secretion in a Ca²⁺ -dependent manner. Inte

SUBMITTER: Cabral-Costa JV 

PROVIDER: S-EPMC10478152 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature