The feasibility of using the compound kushen injection to treat cervical cancer based on network pharmacology and transcriptomics.

ABSTRACT:

Background

To investigate the molecular targets and mechanisms of compound kushen injection (CKI) in the prevention and treatment of cervical cancer based on network pharmacology and transcriptomics.

Methods

In this study, we used network pharmacology methods to screen for effective compounds, integrated the results of network pharmacology and RNA-seq to comprehensively screen and predict target genes, analyze the biological functions and signaling pathways of target genes, and construct a PPI network to screen for hub genes. The results were further verified by biological experiments, molecular docking, RT-PCR, and western blot analysis.

Results

The results showed that the hub genes CXCL2, anti-vascular endothelial growth factor, hexokinase 2 are therapeutic targets

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC10489532 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature