Mechanism of icariin for the treatment of osteoarthritis based on network pharmacology and molecular docking method.

ABSTRACT:

Background

Icarin's mechanism of action in osteoarthritis (OA) was explored using network pharmacology and the GEO database, and then further validated using molecular docking.

Methods

GEO database using network pharmacology identified differential genes in OA based on Icariin's possible targets predicted by pharmmapper database. Combining the differentially expressed genes in OA with the OA-related targets, the overlapping targets were removed. In order to determine what Icariin's core targets are for treating OA, PPI network analysis was performed using OA-related targets and possible Icariin targets. Furthermore, molecular docking was used to verify the chemical's binding to the targets. Final steps included Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of

SUBMITTER: Gu JY 

PROVIDER: S-EPMC10492078 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature