Phosphoproteomics reveals content and signaling differences between neonatal and adult platelets.

ABSTRACT:

Background and objective

Recent clinical studies have shown that transfusions of adult platelets increase morbidity and mortality in preterm infants. Neonatal platelets are hyporesponsive to agonist stimulation, and emerging evidence suggests developmental differences in platelet immune functions. This study was designed to compare the proteome and phosphoproteome of resting adult and neonatal platelets.

Methods

We isolated resting umbilical cord blood-derived platelets from healthy full term neonates (n=9) and resting blood platelets from healthy adults (n=7), and compared protein and phosphoprotein contents using data independent acquisition mass spectrometry.

Results

We identified 4745 platelet proteins with high confidence across all samples. Adult and neonatal pl

SUBMITTER: Thom CS 

PROVIDER: S-EPMC10515911 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature