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Red-Shifted Coumarin Luciferins for Improved Bioluminescence Imaging.

ABSTRACT: Multicomponent bioluminescence imaging in vivo requires an expanded collection of tissue-penetrant probes. Toward this end, we generated a new class of near-infrared (NIR) emitting coumarin luciferin analogues (CouLuc-3s). The scaffolds were easily accessed from commercially available dyes. Complementary mutant luciferases for the CouLuc-3 analogues were also identified. The brightest probes enabled sensitive imaging in vivo. The CouLuc-3 scaffolds are also orthogonal to popular bioluminescent reporters and can be used for multicomponent imaging applications. Collectively, this work showcases a new set of bioluminescent tools that can be readily implemented for multiplexed imaging in a variety of biological settings.

SUBMITTER: Love AC

PROVIDER: S-EPMC10519142 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature

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Red-Shifted Coumarin Luciferins for Improved Bioluminescence Imaging.

Love Anna C AC Caldwell Donald R DR Kolbaba-Kartchner Bethany B Townsend Katherine M KM Halbers Lila P LP Yao Zi Z Brennan Caroline K CK Ivanic Joseph J Hadjian Tanya T Mills Jeremy H JH Schnermann Martin J MJ Prescher Jennifer A JA

Journal of the American Chemical Society 20230206 6

Multicomponent bioluminescence imaging in vivo requires an expanded collection of tissue-penetrant probes. Toward this end, we generated a new class of near-infrared (NIR) emitting coumarin luciferin analogues (CouLuc-3s). The scaffolds were easily accessed from commercially available dyes. Complementary mutant luciferases for the CouLuc-3 analogues were also identified. The brightest probes enabled sensitive imaging in vivo. The CouLuc-3 scaffolds are also orthogonal to popular bi ...[more]

PMID: 36745536

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