Molecular Evidence of Structural Changes in Silk Using Unlimited Degradation Mass Spectrometry.

ABSTRACT: Proteomics has important uses in archeological science because it can distinguish species, reveal the evolution of paleontology, and provide biological evidence of historical events. However, this technique still has full potential in the study of silk aging mechanisms. In this work, we propose a strategy combining unlimited degradation with mass-spectrometry-based proteomics techniques, which interpret protein fragmentation propensity and secondary structure changes by detecting content changes of specific peptide groups in complex proteomes. This approach was employed to study the conformational changes in silk microscopic crystals after heat treatment. Combining conventional mechanics and crystallographic characterization, a thermal aging degradation mechanism model was proposed. At the

SUBMITTER: Zhou J 

PROVIDER: S-EPMC10536863 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature