Immunomodulation of the donor lung with CRISPR-mediated activation of IL-10 expression.

ABSTRACT:

Background

Inflammatory injury in the donor lung remains a persistent challenge in lung transplantation that limits donor organ usage and post-transplant outcomes. Inducing immunomodulatory capacity in donor organs could address this unsolved clinical problem. We sought to apply clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) technologies to the donor lung to fine-tune immunomodulatory gene expression, exploring for the first time the therapeutic use of CRISPR-mediated transcriptional activation in the whole donor lung.

Methods

We explored the feasibility of CRISPR-mediated transcriptional upregulation of interleukin 10 (IL-10), a key immunomodulatory cytokine, in vitro and in vivo. We first evaluated the potency, titratability, and multip

SUBMITTER: Mesaki K 

PROVIDER: S-EPMC10538378 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature