Xenograft Skin Model to Manipulate Human Immune Responses In Vivo.

ABSTRACT: The human skin xenograft model, in which human donor skin is transplanted onto an immunodeficient mouse host, is an important option for translational research in skin immunology. Murine and human skin differ substantially in anatomy and immune cell composition. Therefore, traditional mouse models have limitations for dermatological research and drug discovery. However, successful xenotransplants are technically challenging and require optimal specimen and mouse graft site preparation for graft and host survival. The present protocol provides an optimized technique for transplanting human skin onto mice and discusses necessary considerations for downstream experimental aims. This report describes the appropriate preparation of a human donor skin sample, assembly of a surgical setup, mouse

SUBMITTER: Moss MI 

PROVIDER: S-EPMC10552904 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature