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A natural mutation in the promoter of Ms-cd1 causes dominant male sterility in Brassica oleracea.

ABSTRACT: Male sterility has been used for crop hybrid breeding for a long time. It has contributed greatly to crop yield increase. However, the genetic basis of male sterility has not been fully elucidated. Here, we report map-based cloning of the cabbage (Brassica oleracea) dominant male-sterile gene Ms-cd1 and reveal that it encodes a PHD-finger motif transcription factor. A natural allele Ms-cd1_PΔ-597, resulting from a 1-bp deletion in the promoter, confers dominant genic male sterility (DGMS), whereas loss-of-function ms-cd1 mutant shows recessive male sterility. We also show that the ethylene response factor BoERF1L represses the expression of Ms-cd1 by directly binding to its promoter; however, the 1-bp deletion in Ms-cd1_PΔ-597 affects the binding. Furthermore, ectopic expression of Ms-cd1_PΔ-597 confers DGMS in both dicotyledonous and monocotyledonous plant species. We thus propose that the DGMS system could be useful for breeding hybrids of multiple crop species.

SUBMITTER: Han F

PROVIDER: S-EPMC10556095 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature

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A natural mutation in the promoter of Ms-cd1 causes dominant male sterility in Brassica oleracea.

Han Fengqing F Yuan Kaiwen K Sun Wenru W Zhang Xiaoli X Liu Xing X Zhao Xinyu X Yang Limei L Wang Yong Y Ji Jialei J Liu Yumei Y Li Zhansheng Z Zhang Jinzhe J Zhang Chunzhi C Huang Sanwen S Zhang Yangyong Y Fang Zhiyuan Z Lv Honghao H

Nature communications 20231005 1

Male sterility has been used for crop hybrid breeding for a long time. It has contributed greatly to crop yield increase. However, the genetic basis of male sterility has not been fully elucidated. Here, we report map-based cloning of the cabbage (Brassica oleracea) dominant male-sterile gene Ms-cd1 and reveal that it encodes a PHD-finger motif transcription factor. A natural allele Ms-cd1<sub>PΔ-597,</sub> resulting from a 1-bp deletion in the promoter, confers dominant genic male sterility (DG ...[more]

PMID: 37798291

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Project description:BACKGROUND: The novel chimeric open reading frame (orf) resulting from the rearrangement of a mitochondrial genome is generally thought to be a causal factor in the occurrence of cytoplasmic male sterility (CMS). Both positive and negative correlations have been found between CMS-associated orfs and the occurrence of CMS when CMS-associated orfs were expressed and targeted at mitochondria. Some orfs cause male sterility or semi-sterility, while some do not. Little is currently known about how mitochondrial factor regulates the expression of the nuclear genes involved in male sterility. The purpose of this study was to investigate the biological function of a candidate CMS-associated orf220 gene, newly isolated from cytoplasmic male-sterile stem mustard, and show how mitochondrial retrograde regulated nuclear gene expression is related to male sterility. RESULTS: It was shown that the ORF220 protein can be guided to the mitochondria using the mitochondrial-targeting sequence of the ? subunit of F1-ATPase (atp2-1). Transgenic stem mustard plants expressed the chimeric gene containing the orf220 gene and a mitochondrial-targeting sequence of the ? subunit of F1-ATPase (atp2-1). Transgenic plants were male-sterile, most being unable to produce pollen while some could only produce non-vigorous pollen. The transgenic stem mustard plants also showed aberrant floral development identical to that observed in the CMS stem mustard phenotype. Results obtained from oligooarray analysis showed that some genes related to mitochondrial energy metabolism were down-regulated, indicating a weakening of mitochondrial function in transgenic stem mustard. Some genes related to pollen development were shown to be down-regulated in transgenic stem mustard and the expression of some transcription factor genes was also altered. CONCLUSION: The work presented furthers our understanding of how the mitochondrially-targeted expression of CMS-associated orf220 gene causes male sterility through retrograde regulation of nuclear gene expression in Brassica juncea.

Dataset Information

A natural mutation in the promoter of Ms-cd1 causes dominant male sterility in Brassica oleracea.

Publications

A natural mutation in the promoter of Ms-cd1 causes dominant male sterility in Brassica oleracea.

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