Insulin-induced gene 2 protects against hepatic ischemia-reperfusion injury via metabolic remodeling.

ABSTRACT:

Background

Hepatic ischemia-reperfusion (IR) injury is the primary reason for complications following hepatectomy and liver transplantation (LT). Insulin-induced gene 2 (Insig2) is one of several proteins that anchor the reticulum in the cytoplasm and is essential for metabolism and inflammatory responses. However, its function in IR injury remains ambiguous.

Methods

Insig2 global knock-out (KO) mice and mice with adeno-associated-virus8 (AAV8)-delivered Insig2 hepatocyte-specific overexpression were subjected to a 70% hepatic IR model. Liver injury was assessed by monitoring hepatic histology, inflammatory responses, and apoptosis. Hypoxia/reoxygenation stimulation (H/R) of primary hepatocytes and hypoxia model induced by cobalt chloride (CoCl₂) were used for in

SUBMITTER: Wu Y 

PROVIDER: S-EPMC10585752 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature