High-Temporal-Resolution smFISH Method for Gene Expression Studies in Caenorhabditis elegans Embryos.

ABSTRACT: Recent development in fluorescence-based molecular tools has contributed significantly to developmental studies, including embryogenesis. Many of these tools rely on multiple steps of sample manipulation, so obtaining large sample sizes presents a major challenge as it can be labor-intensive and time-consuming. However, large sample sizes are required to uncover critical aspects of embryogenesis, for example, subtle phenotypic differences or gene expression dynamics. This problem is particularly relevant for single-molecule fluorescence in situ hybridization (smFISH) studies in Caenorhabditis elegans embryogenesis. Microfluidics can help address this issue by allowing a large number of samples and parallelization of experiments. However, performing efficient reagent exchange on chip

SUBMITTER: Charles S 

PROVIDER: S-EPMC10619480 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature