Subvacuum environment-enhanced cell migration promotes wound healing without increasing hypertrophic scars caused by excessive cell proliferation.

ABSTRACT: Cell migration and proliferation are conducive to wound healing; however, regulating cell proliferation remains challenging, and excessive proliferation is an important cause of scar hyperplasia. Here, we aimed to explore how a subvacuum environment promotes wound epithelisation without affecting scar hyperplasia. Human immortalized keratinocyte cells and human skin fibroblasts were cultured under subvacuum conditions (1/10 atmospheric pressure), and changes in cell proliferation and migration, target protein content, calcium influx, and cytoskeleton and membrane fluidity were observed. Mechanical calcium (Ca²⁺ ) channel blockers were used to prevent Ca²⁺ influx for reverse validation. A rat wound model was used to elucidate the mechanism of the subvacuum dressing in

SUBMITTER: Jin J 

PROVIDER: S-EPMC10623940 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature