ASMT determines gut microbiota and increases neurobehavioral adaptability to exercise in female mice.

ABSTRACT: N-acetylserotonin O-methyltransferase (ASMT) is responsible for melatonin biosynthesis. The Asmt gene is located on the X chromosome, and its genetic polymorphism is associated with depression in humans. However, the underlying mechanism remains unclear. Here, we use CRISPR/Cas9 to delete 20 bp of exon 2 of Asmt, and construct C57BL/6J mouse strain with Asmt frameshift mutation (Asmt^ft/ft). We show that female Asmt^ft/ft mice exhibit anxiety- and depression-like behaviors, accompanied by an obvious structural remodeling of gut microbiota. These behavioral abnormalities are not observed in male. Moreover, female Asmt^ft/ft mice show a lower neurobehavioral adaptability to exercise, while wild-type shows a "higher resilience". Cross-sectional and longitudinal a

SUBMITTER: Liu W 

PROVIDER: S-EPMC10630421 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature