Simple Protein Analysis by Droplet Paper Spray Ionization Mass Spectrometry with Polyolefin Silica-Based Paper.

ABSTRACT: Paper spray ionization mass spectrometry (PSI MS) has emerged as a notable method for the rapid analysis of biological samples. However, the typical cellulose-based paper tip is incompatible with protein detection due to the strong interaction between cellulose hydroxyl groups and proteins. In this study, we utilized a commercially available polyolefin-based synthetic paper, Teslin^®, as an alternative PSI substrate for simple protein analysis. We have named this method "droplet PSI" MS, as the aqueous protein solution droplet retains its shape on the Teslin^® paper tip. For droplet PSI, no further chemical pretreatment was necessary for the Teslin^® substrate; the only required preparation was shaping the Teslin^® paper into a triangular tip. In dro

SUBMITTER: An SJ 

PROVIDER: S-EPMC10647424 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature