Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Achieving high hybridization density at DNA biosensor surfaces using branched spacer and click chemistry.

ABSTRACT: The COVID-19 pandemic has highlighted the necessity to develop fast, highly sensitive and selective virus detection methods. Surface-based DNA-biosensors are interesting candidates for this purpose. Functionalization of solid substrates with DNA must be precisely controlled to achieve the required accuracy and sensitivity. In particular, achieving high hybridization density at the sensing surface is a prerequisite to reach a low limit of detection. We herein describe a strategy based on peptides as anchoring units to immobilize DNA probes at the surface of borosilicate slides. While the coating pathway involves copper-catalyzed click chemistry, a copper-free variation is also reported. The resulting biochips display a high hybridization density (2.9 pmol per cm²) with their targeted gene sequences.

SUBMITTER: Kavand A

PROVIDER: S-EPMC10660212 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

Achieving high hybridization density at DNA biosensor surfaces using branched spacer and click chemistry.

Kavand Alireza A Robin Perrine P Mayoraz Lucas L Mensi Mounir M Gerber-Lemaire Sandrine S

RSC advances 20231121 48

The COVID-19 pandemic has highlighted the necessity to develop fast, highly sensitive and selective virus detection methods. Surface-based DNA-biosensors are interesting candidates for this purpose. Functionalization of solid substrates with DNA must be precisely controlled to achieve the required accuracy and sensitivity. In particular, achieving high hybridization density at the sensing surface is a prerequisite to reach a low limit of detection. We herein describe a strategy based on peptides ...[more]

PMID: 38020007

Similar Datasets

The power of microdroplet photochemistry† † Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d4sc00056k

Project description: Not available

| S-EPMC10915808 | biostudies-literature

Total synthesis of bi-magnolignan† † Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d3ra01121f

Project description:Bi-magnolignan, isolated from the leaves of Magnolia officinalis, has shown excellent physiological activity against tumor cells. An efficient strategy for the first total synthesis of bi-magnolignan is reported. The bi-dibenzofuran skeleton was constructed via functional group interconversions of commercially available materials 1,2,4-trimethoxybenzene and 4-allylanisole. Then, the dibenzofuran skeleton was afforded by subsequent Suzuki coupling and intramolecular dehydration. The total synthesis of natural product was accomplished through FeCl3 catalyzed oxidative coupling. The first total synthesis of bi-magnolignan in eight steps from commercially available starting materials has been developed.

| S-EPMC10018648 | biostudies-literature

Boric acid catalysed hydrolysis of peroxyacids† † Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d3ra01046e

Project description:This study shows for the first time that boric acid catalyses the hydrolysis of peroxyacids, resulting in an approximately 12-fold increase in hydrolysis rate for both peracetic acid (PAA) and 3-chloroperbenzoic acid (MCPBA) when 0.1 M boric acid is present. The maximum rate of hydrolysis occurs at pH 9 and pH 8.4 for PAA and MCPBA respectively. In contrast, carbonate buffer does not enhance the rate of PAA hydrolysis. The reaction was followed by measuring the initial rate of hydrogen peroxide formation using a specific Ti(iv) complexation method. The study of the hydrolysis reaction requires the presence of 2 × 10−5 M each of ethylenediaminetetraacetic acid (EDTA) and ethylenediamine tetramethylene phosphonic acid (EDTMP) in all solutions in order to chelate metal ions across the full pH range (3 to 13) that would otherwise contribute to peroxyacid decomposition. Catalysis of peroxyacid hydrolysis is most likely effected by the triganol boric acid acting as a Lewis acid catalyst, associating with the peroxide leaving group in the transition state to reduce the leaving group basicity. The products of the reaction are the well characterised monoperoxoborate species and the parent carboxylic acid. Analysis of the pH and borate dependence data reveals that in addition to a catalytic pathway involving a single boric acid molecule, there is a significant pathway involving either (a) two boric acid molecules or (b) the polyborate species, B3O3(OH)4−. Knowledge about catalytic mechanisms for the loss of peroxyacids through hydrolysis is important because they are widely used in reagents in a range of oxidation, bleaching and disinfection applications. Boric acid catalyses the hydrolysis of peroxyacids, with possible pathways involving one and two molecules of boric acid as well as polyborate species.

| S-EPMC10103745 | biostudies-literature

AI facilitated fluoro-electrochemical phytoplankton classification† † Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d3sc01741a

Project description:Marine phytoplankton is extremely diverse. Counting and characterising phytoplankton is essential for understanding climate change and ocean health not least since phytoplankton extensively biomineralize carbon dioxide whilst generating 50% of the planet's oxygen. We report the use of fluoro-electrochemical microscopy to distinguish different taxonomies of phytoplankton by the quenching of their chlorophyll-a fluorescence using chemical species oxidatively electrogenerated in situ in seawater. The rate of chlorophyll-a quenching of each cell is characteristic of the species-specific structural composition and cellular content. But with increasing diversity and extent of phytoplankton species under study, human interpretation and distinction of the resulting fluorescence transients becomes increasingly and prohibitively difficult. Thus, we further report a neural network to analyse these fluorescence transients, with an accuracy >95% classifying 29 phytoplankton strains to their taxonomic orders. This method transcends the state-of-the-art. The success of the fluoro-electrochemical microscopy combined with AI provides a novel, flexible and highly granular solution to phytoplankton classification and is adaptable for autonomous ocean monitoring. Schematic of fluoro-electrochemical microscopy. (a) Cartoon E. huxleyi is green under normal light, but (b) emits red fluorescence under UV. (c) Placed near an oxidizing electrode, its fluorescence fades and ultimately (d) “switches off”.

| S-EPMC10246652 | biostudies-literature

Electrically conductive [Fe4S4]-based organometallic polymers† † Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d3sc02195e

Project description: Not available

| S-EPMC10599474 | biostudies-literature

Application of a bivalent “click” approach to target tyrosyl-DNA phosphodiesterase 1 (TDP1)† † Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d4md00824c

Project description: Not available

| S-EPMC11843577 | biostudies-literature

Concise two-step chemical synthesis of molnupiravir† † Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d2ra05064a

Project description:A concise synthesis of molnupiravir in a one-pot two-step approach starting from uridine is described. Formally, herein, two sets of one-pot two-reaction steps introducing simplicity for purifications and using chemically available reagents are presented. In this context, molnupiravir was obtained in up to 68% overall yield and multigram-scale. In addition, HPLC analysis showed the molnupiravir purity above 99%. Fast, effective and chromatography column-free chemical synthesis of molnupiravir.

| S-EPMC9585438 | biostudies-literature

Deaminative coupling of benzylamines and arylboronic acids† † Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d2sc06055h

Project description:A metal-free deaminative coupling of non-prefunctionalised benzylamines and arylboronic acids is reported. In this operationally simple reaction, a primary amine in benzylamine is converted into a good leaving group in situ using inexpensive and commercially available isoamyl nitrite as a nitrosating reagent. Lewis-acidic arylboronic acids are shown to replace mineral acids such as HCl or HBF4 that are conventionally used in the preparation of aryl diazonium salts. This unlocked the formation of the corresponding diarylmethanes by forging a new C–C bond in good yields. A metal-free deaminative coupling of non-prefunctionalised benzylamines and arylboronic acids is reported.

| S-EPMC9930926 | biostudies-literature

Pathway-dependent supramolecular polymerization by planarity breaking† † Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d4sc02499k

Project description: Not available

| S-EPMC11253169 | biostudies-literature

Rh-catalyzed alkoxycarbonylation of unactivated alkyl chlorides† † Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d2sc04103k

Project description:A general rhodium-catalyzed selective carbonylative coupling of unactivated alkyl chlorides with aliphatic alcohols or phenols to the corresponding esters is presented for the first time. Crucial for this transformation is the addition of sodium iodide, which provides in situ more active alkyl iodides. In the presence of a Rh(i)-DPPP catalyst system diverse esters (81 examples) including industrially relevant acetates from chloro- and dichloromethane can be prepared in a straightforward manner in up to 95% isolated yield. The used ligand not only affects the selectivity of the carbonylation reaction but also controls the selectivity of the preceding halide exchange step. An efficient and convenient rhodium-catalyzed alkoxycarbonylation of unactivated alkyl chlorides was developed for the first time. More than 80 examples of esters were prepared directly from readily available substrates with often high selectivity.

| S-EPMC9682885 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data