Electrochemical Simulation of Phase I Hepatic Metabolism of Voriconazole Using a Screen-Printed Iron(II) Phthalocyanine Electrode
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PROVIDER: S-EPMC10674253 | biostudies-literature | 2023 Nov
REPOSITORIES: biostudies-literature
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Project description:We describe a tiny 3D-printed polymethyl-methacrylate-based plastic sleeve that houses two disposable screen-printed electrodes (SPE) and enables each of the working electrodes (WEs) to work independently, on a different side of a thin barrier, in its own electrochemical (EC) mini-cell, while the SPE counter and reference units are shared for electroanalysis. Optical and EC performance tests proved that the plastic divider between WE1 and WE2 efficiently inhibited solution mixing between the mini-cells. The two neighboring, independently operating mini-cells enabled matched differential measurements in the same sample solution, a tactic designed for elimination of electrochemical interference in complex samples. In a proof-of-principle glucose biosensor trial, a glucose oxidase-modified WE2 and an unmodified WE1 delivered the EC data for the removal of anodic ascorbic acid (AA) interference simply by subtracting the WE1 (background) current from the analyte-specific WE2 current (from buffered sample solution supplemented with glucose/AA), at an anodic H2O2 detection potential of +1 V. The microfabricated SPE accessory is cheap and easy to make and use. For the many dual electrode SPE strips on the market for multiple analytical targets the new device widens the options for their exploitation in assays of biological and environmental samples with complex matrix compositions and significant risks of interference.
| S-EPMC11426311 | biostudies-literature
Project description:Here, we report on the use of electrochemical methods for the detection of volatiles fatty acids (VFAs), namely acetic acid. We used tetra-tert-butyl phthalocyanine (PcH₂-tBu) as the sensing material and investigated its electroanalytical properties by means of cyclic voltammetry (CV) and square wave voltammetry (SWV). To realize the electrochemical sensing system, the PcH₂-tBu has been dropcast-deposited on carbon (C) orgold (Au)screen-printed electrodes (SPEs) and characterized by cyclic voltammetry and scanning electron microscopy (SEM). The SEM analysis reveals that the PcH₂-tBu forms mainly aggregates on the SPEs. The modified electrodes are used for the detection of acetic acid and present a linear current increase when the acetic acid concentration increases. The Cmodified electrode presents a limit of detection (LOD) of 25.77 mM in the range of 100 mM-400 mM, while the Aumodified electrode presents an LOD averaging 40.89 mM in the range of 50 mM-300 mM. When the experiment is realized in a buffered condition, theCmodified electrode presents a lower LOD, which averagesthe 7.76 mM. A pronounced signal decay attributed to an electrode alteration is observed in the case of the gold electrode. This electrode alteration severely affects the coating stability. This alteration is less perceptible in the case of the carbon electrode.
| S-EPMC5039665 | biostudies-literature
Project description:Simple, rapid, and accurate detection of myoinositol (MI) concentration in blood is crucial in diagnosing polycystic ovary syndrome, neurological disorders, and cancer. A novel electrochemical detection (IED) method was established to quantify MI in human serum using a disposable unmodified screen-printed carbon electrode (SPCE) for the first time. MI was detected indirectly by the reaction product of myoinositol dehydrogenase (IDH) and cofactor β-nicotinamide adenine dinucleotide (NAD+). Good linear calibration curves were obtained at the concentration range from 5.0 μM to 500.0 μM (R 2 = 0.9981) with the lower limits of detection (LOD) and quantification (LOQ) of 1.0 μM and 2.5 μM, respectively. Recoveries were calculated at three spiked concentrations, and the values were between 90.3 and 106%, with relative standard deviation values of 3.2-6.2% for intraday precision and 7.1-9.0% for interday precision. The SPCE-electrochemical biosensor is simple, accurate, and without modification, showing great potential for point-of-care testing (POCT) of serum MI in clinical samples.
| S-EPMC8983225 | biostudies-literature
Project description:Simple, rapid and accurate detection of ethanol concentration in blood is very crucial in the diagnosis and management of potential acute ethanol intoxication patients. A novel electrochemical detection method was developed for the quantification of ethanol in human plasma with disposable unmodified screen-printed carbon electrode (SPCE) without sample preparation procedure. Ethanol was detected indirectly by the reaction product of ethanol dehydrogenase (ADH) and cofactor nicotinamide adenine dinucleotide (NAD(+)). Method validation indicated good quantitation precisions with intra-day and inter-day relative standard deviations of ≤9.4% and 8.0%, respectively. Ethanol concentration in plasma is linear ranging from 0.10 to 3.20 mg/mL, and the detection limit is 40.0 μg/mL (S/N > 3). The method shows satisfactory correlation with the reference method of headspace gas chromatography in twenty human plasma samples (correlation coefficient 0.9311). The proposed method could be applied to diagnose acute ethanol toxicity or ethanol-related death.
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Project description:Electrode interfaces with both antibiofouling properties and electrocatalytic activity can promote the practical application of nonenzymatic electrochemical sensors in biological fluids. Compared with graphene, graphene oxide (GO) possesses unique properties such as superior solubility (hydrophilicity) in water, negative charge, and abundant oxygenated groups (oxo functionalities) in the plane and edge sites, which play an essential role in electrocatalysis and functionalization. In this work, a micro electrochemical sensor consisting of GO-modified screen-printed electrode and PDMS micro-cell was designed to achieve multi-analyte detection with excellent selectivity and anti-biofouling properties by electrochemically tuning the oxygen-containing functional species, hydrophilicity/hydrophobicity, and electrical conductivity. In particular, the presented electrodes demonstrated the potential in the analysis of biological samples in which electrodes often suffer from serious biofouling. The interaction of proteins with electrodes as well as uric acid was investigated and discussed.
| S-EPMC8875984 | biostudies-literature
Project description:A simple, rapid method of the determination of nitrite in food samples is reported by using a highly sensitive electrochemical sensor based on nickel, poly(diallyldimethylammonium chloride) (PDDA), reduced graphene oxide (rGO) and a disposable screen-printed carbon electrode (SPCE). The method is based on a modification of the electrode to enhance the sensitivity and selectivity of the disposable and applicable SPCE, which is essential for the present analytical challenge. The nitrite determination was performed by using a cyclic voltammetry (CV) method under optimum conditions. Ni/PDDA/rGO/SPCE showed a linear working range of 6 to 100 μM of nitrite concentration. The limit of detection and limit of quantification were 1.99 μM (S/N = 3) and 6.6 μM (S/N = 10), respectively. The sensitivities were 0.453 μA μM-1 cm-2 for the lower concentration range and 0.171 μA μM-1 cm-2 for the higher concentration range. The Ni/PDDA/rGO sensor also showed excellent anti-interference ability and good long-term stability. The purposed disposable sensor was successfully applied to determine nitrite in sausages and pickled vegetable samples with satisfactory recovery.
| S-EPMC9562089 | biostudies-literature
Project description:Screen-printed electrochemical sensing platforms, due to their scales of economy and high reproducibility, can provide a useful approach to translate laboratory-based electrochemistry into the field. An important factor when utilising screen-printed electrodes (SPEs) is the determination of their real electrochemical surface area, which allows for the benchmarking of these SPEs and is an important parameter in quality control. In this paper, we consider the use of cyclic voltammetry and chronocoulometry to allow for the determination of the real electrochemical area of screen-printed electrochemical sensing platforms, highlighting to experimentalists the various parameters that need to be diligently considered and controlled in order to obtain useful measurements of the real electroactive area.
| S-EPMC6023085 | biostudies-literature
Project description:An impedance technique-based aptasensor for the detection of thrombin was developed using a single-walled carbon nanotube (SWCNT)-modified screen-printed carbon electrode (SPCE). In this work, a thrombin-binding aptamer (TBA) as probe was used for the determination of thrombin, and that was immobilized on SWCNT through π-π interaction. In the presence of thrombin, the TBA on SWCNT binds with target thrombin, and the amount of TBA on the SWCNT surface decreases. The detachment of TBA from SWCNT will be affected by the concentration of thrombin and the remaining TBA on the SWCNT surface can be monitored by electrochemical methods. The TBA-modified SWCNT/SPCE sensing layer was characterized by cyclic voltammetry (CV). For the measurement of thrombin, the change in charge-transfer resistance (Rct) of the sensing interface was investigated using electrochemical impedance spectroscopy (EIS) with a target thrombin and [Fe(CN)6]3- as redox maker. Upon incubation with thrombin, a decrease of Rct change was observed due to the decrease in the repulsive interaction between the redox marker and the electrode surface without any label. A plot of Rct changes vs. the logarithm of thrombin concentration provides the linear detection ranges from 0.1 nM to 1 µM, with a ~0.02 nM detection limit.
| S-EPMC9002654 | biostudies-literature
Project description:A original electrochemical sensing platform, based on screen-printed electrodes modification with plasma polymerized acrylonitrile (pp-AN) nanofilms is proposed. For that purpose, plasma-enhanced chemical vapor deposition (PECVD) process was conducted in a parallel plate (13.56 MHz) plasma reactor for 2 min with discharge power of 10 W. The surface topography and electrochemical properties of prepared sensors were investigated by X-ray photoelectron spectroscopy, scanning electron microscopy, energy dispersion spectroscopy, electrochemical impedance spectroscopy, and cyclic voltammetry. The electrochemical characteristics of pp-AN/SPCE and pp-AN/SPAuE sensors was investigated for model redox pair [Fe(CN)6]4-/3-. Conducted research confirmed the excellent chemical stability, durability, wide potential window, high signal-to-noise (S/N) ratio, and, most importantly, the ability to standardize the sensors. The pp-AN/SPCE sensor was applied to the determination of bupropion, an antidepressant drug whose intake has increased dramatically during the COVID-19 pandemic. The voltammetric response of pp-AN/SPCE for BUP was linear in two concentration ranges of 0.63-10.0 and 10.0-50.0 μmol L-1, with a detection limit of 0.21 μmol L-1. Satisfactory recoveries (96.2-102%) and good precision (RSD below 4.1%) obtained for environmental and biological samples confirmed the usefulness of the sensor for the analysis of various kinds of samples.
| S-EPMC10499721 | biostudies-literature
Project description:Screen-printed sensors with chemically deposited boron-doped diamond electrodes (BDDE) were modified with different types of gold nanoparticles (AuNPs) according to a new original procedure. Physically and electrochemically deposited AuNPs had various sizes and also nanoporous character. They also differ in shape and density of surface coverage. The developed sensors were characterized using scanning electron microscopy and Raman spectroscopy. Their electrochemical properties were studied using cyclic voltammetry and electrochemical impedance spectrometry of selected outer sphere ([Ru(NH3)6]Cl3) and inner sphere (K3[Fe(CN)6], dopamine) redox markers. The application possibilities of such novel screen-printed sensors with BDDE modified by AuNPs were verified in the analysis of the neurotransmitter dopamine. The best analytical performance was achieved using printed sensors modified with the smallest AuNPs. The achieved limit of detection values in nanomolar concentrations (2.5 nmol L-1) are much lower than those of unmodified electrodes, which confirms the significant catalytic effects of gold nanoparticles on the surface of the working electrode. Sensors with the best electrochemical properties were successfully applied in the analysis of a model solution and spiked urine samples.
| S-EPMC10700314 | biostudies-literature