ThermoCyte: an inexpensive open-source temperature control system for in vitro live-cell imaging.

ABSTRACT: Live-cell imaging is a common technique in microscopy to investigate dynamic cellular behaviour and permits the accurate and relevant analysis of a wide range of cellular and tissue parameters, such as motility, cell division, wound healing responses and calcium (Ca²⁺) signalling in cell lines, primary cell cultures and ex vivo preparations. Furthermore, this can occur under many experimental conditions, making live-cell imaging indispensable for biological research. Systems which maintain cells at physiological conditions outside of a CO₂ incubator are often bulky, expensive and use proprietary components. Here we present an inexpensive, open-source temperature control system for in vitro live-cell imaging. Our system 'ThermoCyte', which is c

SUBMITTER: O'Carroll R 

PROVIDER: S-EPMC10685113 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature