Use of Human Intestinal Enteroids for Recovery of Infectious Human Norovirus from Berries and Lettuce.

ABSTRACT: Norovirus (NoV) is the leading cause of viral foodborne gastroenteritis globally. Currently, the gold standard for detecting NoV in clinical, food, and environmental samples is via molecular-based methods, primarily RT-PCR. Nevertheless, there is a great need for confirmatory assays that can determine the infectivity of viral particles recovered from contaminated matrices. The use of the human intestinal enteroids system (HIEs) has allowed for the expansion of norovirus replication, although it still suffers from limitations of strain preferences and the requirement of high titer stocks for infection. In this study, we wanted to explore the feasibility of using the HIEs to support the replication of NoV that had been recovered from representative food matrices that have been associated wit

SUBMITTER: Wales SQ 

PROVIDER: S-EPMC10706681 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature