Novel sandwich immunoassay detects a shrimp AHPND-causing binary PirAB^Vp toxin produced by Vibrio parahaemolyticus.

ABSTRACT:

Introduction

The binary PirA/PirB toxin expressed by Vibrio parahaemolyticus (PirAB^Vp) is a virulent complex that causes acute hepatopancreatic necrosis disease (AHPND) in shrimps, affecting the global shrimp farming industry. AHPND is currently diagnosed by detecting pirA and pirB genes by PCR; however, several V. parahaemolyticus strains do not produce the two toxins as proteins. Thus, an immunoassay using antibodies may be the most effective tool for detecting toxin molecules. In this study, we report a sandwich ELISA-based immunoassay for the detection of PirAB^Vp.

Methods

We utilized a single-chain variable fragment (scFv) antibody library to select scFvs against the PirA or PirB subunits. Phage display panning rounds wer

SUBMITTER: Jeon MY 

PROVIDER: S-EPMC10711049 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature