3D reconstruction of murine mitochondria reveals changes in structure during aging linked to the MICOS complex.

ABSTRACT: During aging, muscle gradually undergoes sarcopenia, the loss of function associated with loss of mass, strength, endurance, and oxidative capacity. However, the 3D structural alterations of mitochondria associated with aging in skeletal muscle and cardiac tissues are not well described. Although mitochondrial aging is associated with decreased mitochondrial capacity, the genes responsible for the morphological changes in mitochondria during aging are poorly characterized. We measured changes in mitochondrial morphology in aged murine gastrocnemius, soleus, and cardiac tissues using serial block-face scanning electron microscopy and 3D reconstructions. We also used reverse transcriptase-quantitative PCR, transmission electron microscopy quantification, Seahorse analysis, and metabolomics a

SUBMITTER: Vue Z 

PROVIDER: S-EPMC10726809 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature