Ontology highlight
ABSTRACT:
SUBMITTER: Nunthanasup N
PROVIDER: S-EPMC10728061 | biostudies-literature | 2023 Dec
REPOSITORIES: biostudies-literature
Nunthanasup Nuntiporn N Ketprasit Nutpakal N Noulsri Egarit E Palasuwan Attakorn A Combes Valery V Kulkeaw Kasem K Palasuwan Duangdao D
Scientific reports 20231218 1
The use of megakaryoblastic leukemia MEG-01 cells can help reveal the mechanisms of thrombopoiesis. However, conventional in vitro activation of platelet release from MEG-01 cells requires thrombopoietin, which is costly. Here, we aim to develop a more straightforward and affordable method. Synchronization of the MEG-01 cells was initially performed using serum-free culture, followed by spontaneous cell differentiation in the presence of serum. Different stages of megakaryoblast differentiation ...[more]