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Thrombopoietin-independent generation of platelet-like particles from megakaryoblastic cells.


ABSTRACT: The use of megakaryoblastic leukemia MEG-01 cells can help reveal the mechanisms of thrombopoiesis. However, conventional in vitro activation of platelet release from MEG-01 cells requires thrombopoietin, which is costly. Here, we aim to develop a more straightforward and affordable method. Synchronization of the MEG-01 cells was initially performed using serum-free culture, followed by spontaneous cell differentiation in the presence of serum. Different stages of megakaryoblast differentiation were classified based on cell morphology, DNA content, and cell cycle. The MEG-01 cells released platelet-like particles at a level comparable to that of the thrombopoietin-activated MEG-01 cells. The platelet-like particles were distinguishable from PLP-derived extracellular vesicles and could express P-selectin following ADP activation. Importantly, the platelet-like particles induced fibrin clotting in vitro using platelet-poor plasma. Therefore, this thrombopoietin-independent cell synchronization method is an effective and straightforward method for studying megakaryopoiesis and thrombopoiesis.

SUBMITTER: Nunthanasup N 

PROVIDER: S-EPMC10728061 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature

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Thrombopoietin-independent generation of platelet-like particles from megakaryoblastic cells.

Nunthanasup Nuntiporn N   Ketprasit Nutpakal N   Noulsri Egarit E   Palasuwan Attakorn A   Combes Valery V   Kulkeaw Kasem K   Palasuwan Duangdao D  

Scientific reports 20231218 1


The use of megakaryoblastic leukemia MEG-01 cells can help reveal the mechanisms of thrombopoiesis. However, conventional in vitro activation of platelet release from MEG-01 cells requires thrombopoietin, which is costly. Here, we aim to develop a more straightforward and affordable method. Synchronization of the MEG-01 cells was initially performed using serum-free culture, followed by spontaneous cell differentiation in the presence of serum. Different stages of megakaryoblast differentiation  ...[more]

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