Comprehensive characterization of pathogenic synovial fluid extracellular vesicles from knee osteoarthritis.

ABSTRACT: Synovial fluid (SF) extracellular vesicles (EVs) play a pathogenic role in osteoarthritis (OA). However, the surface markers, cell and tissue origins, and effectors of these EVs are largely unknown. We found that SF EVs contained 692 peptides that were positively associated with knee radiographic OA severity; 57.4% of these pathogenic peptides were from 46 proteins of the immune system, predominantly the innate immune system. CSPG4, BGN, NRP1, and CD109 are the major surface markers of pathogenic SF EVs. Genes encoding surface marker CSPG4 and CD109 were highly expressed by chondrocytes from damaged cartilage, while VISG4, MARCO, CD163 and NRP1 were enriched in the synovial immune cells. The frequency of CSPG4⁺ and VSIG4⁺ EV subpopulations in OA SF was high. We conclu

SUBMITTER: Zhang X 

PROVIDER: S-EPMC10735321 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature