Establishment of a juvenile mouse asthma model induced by postnatal hyperoxia exposure combined with early OVA sensitization.

ABSTRACT:

Objective

To establish a juvenile mouse asthma model by postnatal hyperoxia exposure combined with early ovalbumin (OVA) sensitization.

Methods

Female C57BL/6J newborn mice were exposed to hyperoxia (95 % O₂) from postnatal day-1 (PND1) to PND7; intraperitoneally injected with OVA suspension on PND21, PND28; and stimulated by nebulized inhalation of 1 % OVA from PND36 to PND42. Within 48 h of the last challenge, we observed their activity performance and evaluated airway responsiveness (AHR). All mice were executed at PND44. Female (n = 32) were divided into four groups as follows: room air（RA）+phosphate-buffered saline (PBS) group; O₂ (hyperoxia, 95 % O₂) + PBS group; RA + OVA group; O₂+OVA group. We obtained the serum, bronchoalv

SUBMITTER: Li J 

PROVIDER: S-EPMC10750071 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature