Lineage tracing of T cell differentiation from T-iPSC by 2D feeder-free culture and 3D organoid culture.

ABSTRACT:

Introduction

T cells induced from induced pluripotent stem cells(iPSCs) derived from antigen-specific T cells (T-iPS-T cells) are an attractive tool for T cell immunotherapy. The induction of cytotoxic T-iPS-T cells is well established in feeder-free condition for the aim of off-the-shelf production, however, the induction of helper T-iPS-T cells remains challenging.

Methods

We analyzed T-iPS-T cells matured in 3D organoid culture at different steps in the culture process at the single-cell level. T-iPS-T cell datasets were merged with an available human thymocyte dataset based in single-cell RNA sequencing (scRNA-seq). Particularly, we searched for genes crucial for generation CD4+ T-iPS-T cells by comparing T-iPS-T cells established in 2D feeder-free or 3D organoid culture

SUBMITTER: Ishiguro Y 

PROVIDER: S-EPMC10757342 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature