Phospholipase C-γ as a Potential Therapeutic Target for Graves' Orbitopathy.

ABSTRACT:

Backgruound

Phospholipase C-γ (PLC-γ) plays a crucial role in immune responses and is related to the pathogenesis of various inflammatory disorders. In this study, we investigated the role of PLC-γ and the therapeutic effect of the PLC-specific inhibitor U73122 using orbital fibroblasts from patients with Graves' orbitopathy (GO).

Methods

The expression of phospholipase C gamma 1 (PLCG1) and phospholipase C gamma 2 (PLCG2) was evaluated using polymerase chain reaction in GO and normal orbital tissues/fibroblasts. The primary cultures of orbital fibroblasts were treated with non-toxic concentrations of U73122 with or without interleukin (IL)-1β to determine its therapeutic efficacy. The proinflammatory cytokine levels and activation of downstream signaling molecules were dete

SUBMITTER: Roh TH 

PROVIDER: S-EPMC10765002 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature