Delineating endogenous Cushing's syndrome by GC-MS urinary steroid metabotyping.

ABSTRACT:

Background

Diagnosing Cushing's syndrome (CS) is highly complex. As the diagnostic potential of urinary steroid metabolome analysis by gas chromatography-mass spectrometry (GC-MS) in combination with systems biology has not yet been fully exploited, we studied a large cohort of patients with CS.

Methods

We quantified daily urinary excretion rates of 36 steroid hormone metabolites. Applying cluster analysis, we investigated a control group and 168 patients: 44 with Cushing's disease (CD) (70% female), 18 with unilateral cortisol-producing adrenal adenoma (83% female), 13 with primary bilateral macronodular adrenal hyperplasia (PBMAH) (77% female), and 93 ruled-out CS (73% female).

Findings

Cluster-Analysis delineated five urinary steroid metabotypes in CS. Metabotypes

SUBMITTER: Braun LT 

PROVIDER: S-EPMC10776922 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature