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A metabolomics study delineating geographical location-associated primary metabolic changes in the leaves of growing tobacco plants by GC-MS and CE-MS.

ABSTRACT: Ecological conditions and developmental senescence significantly affect the physiological metabolism of plants, yet relatively little is known about the influence of geographical location on dynamic changes in plant leaves during growth. Pseudotargeted gas chromatography-selected ion monitoring-mass spectrometry and capillary electrophoresis-mass spectrometry were used to investigate a time course of the metabolic responses of tobacco leaves to geographical location. Principal component analysis revealed obvious metabolic discrimination between growing districts relative to cultivars. A complex carbon and nitrogen metabolic network was modulated by environmental factors during growth. When the Xuchang and Dali Districts in China were compared, the results indicated that higher rates of photosynthesis, photorespiration and respiration were utilized in Xuchang District to generate the energy and carbon skeletons needed for the biosynthesis of nitrogen-containing metabolites. The increased abundance of defense-associated metabolites generated from the shikimate-phenylpropanoid pathway in Xuchang relative to Dali was implicated in protection against stress.

SUBMITTER: Zhao Y

PROVIDER: S-EPMC4637841 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature

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A metabolomics study delineating geographical location-associated primary metabolic changes in the leaves of growing tobacco plants by GC-MS and CE-MS.

Zhao Yanni Y Zhao Jieyu J Zhao Chunxia C Zhou Huina H Li Yanli Y Zhang Junjie J Li Lili L Hu Chunxiu C Li Wenzheng W Peng Xiaojun X Lu Xin X Lin Fucheng F Xu Guowang G

Scientific reports 20151109

Ecological conditions and developmental senescence significantly affect the physiological metabolism of plants, yet relatively little is known about the influence of geographical location on dynamic changes in plant leaves during growth. Pseudotargeted gas chromatography-selected ion monitoring-mass spectrometry and capillary electrophoresis-mass spectrometry were used to investigate a time course of the metabolic responses of tobacco leaves to geographical location. Principal component analysis ...[more]

PMID: 26549189

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Project description:In order to conduct in-depth research on the stylistic characteristics of different varieties of cigar tobacco leaves (CTLs), we have carried out the following research work: first, the Soxhlet extraction method was utilized for the pretreatment of 4 different varieties of CTLs currently available in China. Then, the two-dimensional (2D) gas chromatography-time-of-flight mass spectrometry (GC × GC-TOF-MS) technology was applied for the nontargeted flavoromics analysis of CTLs. A total of 2117 volatile compounds were identified in the extracts of the 4 CTLs varieties, encompassing alcohols, aldehydes, ketones, carboxylic acids, and their derivatives, esters, hydrocarbons, alkenes, heterocyclic compounds, and aromatics. Results from multivariate statistical analyses (principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA)) revealed significant differences in the volatile compound profiles among different CTL varieties. Based on the relative odor activity value (ROAV) analysis, key flavor compounds were determined for each variety, contributing to the unique aroma profiles of the cigars. Overall, the 4 CTLs shared similar flavor profiles, characterized by sweetness, fruitiness, greenness, nuttiness, and floral notes, with BX1 and DX2 particularly standing out in sweetness and BX1 additionally prominent in fruitiness. HY101, however, was relatively lacking in these flavor attributes compared to the other 3 varieties. Nine key differential compounds-1-butanol, 3-methyl; 1-hexanol; benzeneacetaldehyde; naphthalene; benzaldehyde; isophorone; 2-heptanone, furan, 2-pentyl; and pyrazine, trimethyl, and trimethylpyrazine-were identified as the characteristic markers among different CTL varieties. We believe that this study will not only provide important value for the analysis of flavor characteristics and variety identification of cigar tobacco leaves but also offer scientific basis for improving the quality and market advantages of cigar tobacco leaves.

Dataset Information

A metabolomics study delineating geographical location-associated primary metabolic changes in the leaves of growing tobacco plants by GC-MS and CE-MS.

Publications

A metabolomics study delineating geographical location-associated primary metabolic changes in the leaves of growing tobacco plants by GC-MS and CE-MS.

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