CRISPR-Cas9-mediated IncF plasmid curing in extraintestinal pathogenic Escherichia coli.
Ontology highlight
ABSTRACT: Importance
Understanding the role of IncF plasmids in the success of drug-resistant bacteria has far-reaching implications for tackling antibiotic resistance. The study's use of a novel CRISPR-Cas9-mediated plasmid-curing system provides a precision tool for dissecting the specific impact of IncF plasmids on ExPEC clones, especially high-risk, multidrug-resistant strains like ST131, ST1193, and ST410. The study offers a crucial stepping stone for future research into understanding how these plasmids influence more complex aspects of bacterial behavior, such as cell invasion and in vivo fitness.
SUBMITTER: Chen L
PROVIDER: S-EPMC10783119 | biostudies-literature | 2024 Jan
REPOSITORIES: biostudies-literature
Publications
CRISPR-Cas9-mediated IncF plasmid curing in extraintestinal pathogenic <i>Escherichia coli</i>.
Chen Liang L Peirano Gisele G Yen Kelly K Wang Bingjie B Terlecky Austin A DeVinney Rebekah R Kreiswirth Barry N BN Pitout Johann D D JDD
Microbiology spectrum 20231129 1
<h4>Importance</h4>Understanding the role of IncF plasmids in the success of drug-resistant bacteria has far-reaching implications for tackling antibiotic resistance. The study's use of a novel CRISPR-Cas9-mediated plasmid-curing system provides a precision tool for dissecting the specific impact of IncF plasmids on ExPEC clones, especially high-risk, multidrug-resistant strains like ST131, ST1193, and ST410. The study offers a crucial stepping stone for future research into understanding how th ...[more]
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