M⁶A Profile Dynamics Indicates Regulation of Oyster Development by m⁶A-RNA Epitranscriptomes.

ABSTRACT: The N⁶-methylation of RNA adenosines (N⁶-methyladenosine, m⁶A) is an important regulator of gene expression with critical implications in vertebrate and insect development. However, the developmental significance of epitranscriptomes in lophotrochozoan organisms remains unknown. Using methylated RNA immunoprecipitation sequencing (MeRIP-seq), we generated transcriptome-wide m⁶A-RNA methylomes covering the entire development of the oyster from oocytes to juveniles. Oyster RNA classes display specific m⁶A signatures, with messenger RNAs (mRNAs) and long non-coding RNAs (lncRNAs) exhibiting distinct profiles and being highly methylated compared to transposable element (TE) transcripts. Epitranscriptomes are dynamic and correspond to the c

SUBMITTER: Le Franc L 

PROVIDER: S-EPMC10787124 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature