Intact proviruses are enriched in the colon and associated with PD-1⁺TIGIT^- mucosal CD4⁺ T cells of people with HIV-1 on antiretroviral therapy.

ABSTRACT:

Background

The persistence of intact replication-competent HIV-1 proviruses is responsible for the virological rebound off treatment. The gut could be a major reservoir of HIV-1 due to the high number of infected target cells.

Methods

We collected blood samples and intestinal biopsies (duodenum, ileum, colon) from 42 people with HIV-1 receiving effective antiretroviral therapy. We used the Intact Proviral DNA Assay to estimate the frequency of intact HIV-1 proviruses in the blood and in the intestinal mucosa of these individuals. We analyzed the genetic complexity of the HIV-1 reservoir by performing single-molecule next-generation sequencing of HIV-1 env DNA. The activation/exhaustion profile of mucosal T lymphocytes was assessed by flow cytometry.

Findings

Intact pr

SUBMITTER: Vellas C 

PROVIDER: S-EPMC10792747 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature